Projects/sams2
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SAMS2 Sensor Assay Microbe Systems
This project is in Collaboration with HEIG-VD.
Collaborator
About
SAMS2 is a tabletop system indent to control and operate the two-layered PDMS based microfluidic chip developed at the EPFL (École Polytechnique Fédérale de Lausanne) and the UNIL (University of Lausanne). It controls the cells and nutrient flow and allows operating the valves integrated into the PDMS. All pressures are supervised by the integrated microprocessor
Measurements
The goal of this project is to use genetically modified E. Coli bacteria which can be cultivated and maintained, in the micro-chemostat integrated in the PDMS chip, for several days. With its help a continuously supply of fresh cells is provided. Once a measurement wants to be made, the cells will be pushed into the cells trap where the measurement will be made. Multiple measurements can be done with a single PDMS chip.
Device
- 3 adjustable pressure chambers between 0.1 – 2 bar
- 3 liquid container for nutrient, cells as well as water sample
- 7 user controllable high speed valves to change water sample pressure or open / close channels
- Valve switching time within ~1ms
- USB connection to a computer
- Powered with a microprocessor TI MSP430
- Genetically modified E. coli producing EGFP protein in presence of arsenic
Active Collab
The Collaboration is done on the Active Collab Platform SAMS2 Active Collab
